VARIOUS STAINS USED IN MEDICAL PATHOLOGY

1) HEMATOXYLIN Stains:-

#Blue and basic

#Stains NEGATIVELY CHARGED substances e.g. DNA and RNA

#Nucleus stained.

2) EOSIN Stains:-

#Pink and acidic.

#Stains POSITIVELY CHARGED structures e.g. mitochondria

#Cytoplasm is stained.

3) Oil red O Stains:-

#fat/lipid (on frozen section)

■ other stains for lipid /fat are:

a) Sudan black B
b) Sudan IV
c) Annexin V
d) Osmium teroxide

4) Pas Stains;-

a) Glycogen
b) Fungus and amoeba
c) Basement membrane
d) Lymphoblast (BLOCK LIKE POSITIVITY)
e) Erythroblast (DIFFUSELY POSITIVE)

5) CALCIUM stains:-

a) Von kossa
b) Alizarin red
c) Calcein

6) PERL’S PRUSSIAN BLUE :-

#Iron/hemosiderin

7) FUNGUS stains:-

a) Gomori’s methenamine silver stains —(best fungal stain; stains both live and dead fungus)

b) PAS stain—-only live fungus staining

8) AMYLOID Stains:-

a) Lugol’s iodine—gross specimen
b) Hematoxylin and eosin
c) Methyl violet
d) Crystal violet
e) Thioflavin T
f) Toluidine blue
g) PAS
h) Alcian blue
i) CONGO RED STAIN— pink/red under ordinary light
And APPLE –GREEN BIREFRINGERENCE on POLARISED MICROSCOPY(DIAGNOSTIC)

9) ALCIAN BLUE Stains;-

#Mucin and mucosubstances

10) ACID FAST Stains;-

#Mycobacterium

11) FONTANA MASSON:-

#Melanin and Argentaffin cells.

12) MASSON’s TRICHROME Stains:-

#Collagen and connective tissue

13) VERHOFF-VON-GIESON (VVG):-

#Elastic fibres ( other stain—ORCEIN stain)

14) TOLUIDINE BLUE:-

#Mast cells

15) LUXOL FAST BLUE:-

#Myelin

16) BIELSCHOWSKY Stains:-

(uses silver stain)
#Neurofibrillary tangles and neuritic plaques

Thank you 🙏🤝👍

Source;- MDSMAFE

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